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. 2007 Nov 26;149(3):877–885. doi: 10.1210/en.2007-0893

Table 1.

TaqMan primers and probes for real-time PCR

Sequence
ObR
 5′ Primer CCATCTTTTATATGATCTGCCTGAAGT
 3′ Primer TGCATTGGACAGTCTGAAAGCT
 Probe 6FAM-AGATGATTCGCCTCTGCCCCCACT-TAMRA
ObRa
 5′ Primer GAAGTCTCTCATGACCACTACAGATGA
 3′ Primer TTGTTTCCCTCCATCAAAATGTAA
 Probe 6FAM-CCCAATCTACCAACTTCCCAACAGTCCA-TAMRA
ObRb
 5′ Primer GCATGCAGAATCAGTGATATTTGG
 3′ Primer CAAGCTGTATCGACACTGATTTCTTC
 Probe 6FAM-CCTCTTCTTCTGGAGCCTGAACCCATTTC-TAMRA
gp130
 5′ Primer CCAGCAACGAGGAGAATGAGT
 3′ Primer TGTGCACCACAGTGGAGTACTG
 Probe 6FAM-TCAGAGCACCGCCAGCACGG-TAMRA
GAPDH
 5′ Primer TGTGTCCGTCGTGGATCTGA
 3′ Primer CCTGCTTCACCACCTTCTTGA
 Probe 6FAM-CCGCCTGGAGAAACCTGCCAAGTATG-TAMRA