Table 1.
Sequence | |
---|---|
ObR | |
5′ Primer | CCATCTTTTATATGATCTGCCTGAAGT |
3′ Primer | TGCATTGGACAGTCTGAAAGCT |
Probe | 6FAM-AGATGATTCGCCTCTGCCCCCACT-TAMRA |
ObRa | |
5′ Primer | GAAGTCTCTCATGACCACTACAGATGA |
3′ Primer | TTGTTTCCCTCCATCAAAATGTAA |
Probe | 6FAM-CCCAATCTACCAACTTCCCAACAGTCCA-TAMRA |
ObRb | |
5′ Primer | GCATGCAGAATCAGTGATATTTGG |
3′ Primer | CAAGCTGTATCGACACTGATTTCTTC |
Probe | 6FAM-CCTCTTCTTCTGGAGCCTGAACCCATTTC-TAMRA |
gp130 | |
5′ Primer | CCAGCAACGAGGAGAATGAGT |
3′ Primer | TGTGCACCACAGTGGAGTACTG |
Probe | 6FAM-TCAGAGCACCGCCAGCACGG-TAMRA |
GAPDH | |
5′ Primer | TGTGTCCGTCGTGGATCTGA |
3′ Primer | CCTGCTTCACCACCTTCTTGA |
Probe | 6FAM-CCGCCTGGAGAAACCTGCCAAGTATG-TAMRA |