Fig. 6.

Effects of Ac-SDKP on ET-1-induced MAPK ERK1/2 phosphorylation in rat CFs. CFs were stimulated with ET-1 (1 nM) in the absence or presence of Ac-SDKP (10 nM) at 37°C for 5 min. Top: Western blots of phosphorylated and total ERK1/2. Bottom: semi-quantitative data as % of control from triplicate blots for each protocol. Note that ET-1 markedly increased ERK1/2 phosphorylation, which was attenuated by pretreatment of cells with Ac-SDKP (10 nM). Total ERK1/2 was not changed by any treatment and also served to confirm equal protein loading. Note that, in double bands, bottom represents ERK2 (p42) and top band represents ERK1 (p44). p, Phosphorylated ERK1/2; t, total ERK1/2. *P < 0.05 and **P < 0.01 vs. control; #P < 0.05 vs. ET-1.