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Effects of inhibition of phospholipase C with U-73122 (1 μM) on ANG IV-induced ERK1/2 phosphorylation in rat mesangial cells. Top: Western blots of phosphorylated and total ERK1/2. Bottom: semiquantitative levels of p-ERK1/2. ANG IV significantly increased ERK1/2 phosphorylation, and the effect was blocked by U-73122, suggesting involvement of phospholipase C (n = 6 each). **P < 0.01 vs. control. ##P < 0.01 vs. ANG IV.
