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. Author manuscript; available in PMC: 2009 Apr 18.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 7;368(4):846–851. doi: 10.1016/j.bbrc.2008.01.144

Fig. 3.

Fig. 3

Histone H2BV co-immunoprecipitates with histone H3 that is trimethylated at lysines 4 and 76. (A) A specific antibody was raised against H3K4me3. Pre-incubation of the antibody without peptide or 10 ng/ml H3K4me0, H3K4me3, H3K76me0, or H3K76me3 peptides shows that the antibody only binds the H3K4me3 peptide. An antibody to H4 (Sigma) was used as a loading control. (B) Mononucleosomes containing either H2B-FLAG or H2BV-FLAG were immunoprecipitated with FLAG antibody affinity gel and analyzed by Western blot with H3K4me3 and H3K76me3 antibodies. An antibody to H3 (Abcam ab1791) was used as a loading control.