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. Author manuscript; available in PMC: 2008 Mar 31.
Published in final edited form as: Endocrinology. 2006 Nov 30;148(3):1235–1245. doi: 10.1210/en.2006-1250

Fig. 4.

Fig. 4

Differentiation status on Hoxb13 expression in NRP-152 cells. A, NRP-152 cells grown in GM2 medium with 5% serum. Immunocytochemistry (ICC) staining of p63 (top) and CK8 (bottom) reveal a basal cell phenotype and absence of luminal cells under nondifferentiating growth condition. B, ICC of Hoxb13 protein in lentiviral infected NRP-152 cells. No Hoxb13 protein was found in pHR3-CMV-EGFP virus-infected cells (top). In NRP-152 cells infected with pHR3-CMV-Hoxb13 virus, Hoxb13 protein was localized to nuclei of infected cells (bottom). C, RT-PCR analysis of mRNA from lentiviral-infected NRP-152 cells. Hoxb13 mRNA was not expressed in pHR3-CMV-EGFP virus-infected NRP-152 cells, whereas Hoxb13 mRNA was expressed at high levels in pHR3-CMV-Hoxb13 virus-infected cells (scale bar, 100 μm).