TABLE 1.
Primers and Taqman probes used for real-time PCR
| Gene | Sequence | Amplicon size (bp) |
|---|---|---|
| Hoxa9 | ||
| Forward primer | aggaagagtaccgaggcaag | 159 |
| Reverse primer | taaatcactccgcacgctat | |
| Probe | ctgcgcgatccctttgcataaa | |
| Hoxa10 | ||
| Forward primer | aggactccctgggcaattc | 83 |
| Reverse primer | gtaagggcagcgtttcttcc | |
| Hoxa11 | ||
| Forward primer | gatctgcacccaaacctgag | 159 |
| Reverse primer | caaccagcaaaacctctgga | |
| Probe | ttctacctcccaatggtggtgatgg | |
| Hoxa13 | ||
| Forward primer | ctccccacctctggaagtc | 139 |
| Reverse primer | ttcgtagcgtattcccgttc | |
| Hoxb13 | ||
| Forward primer | gatgtgctgccaaggtgaac | 83 |
| Reverse primer | gaggagggtgctggacac | |
| Probe | aaagcagcgtttgcagagcc | |
| Hoxd13 | ||
| Forward primer | tagccaacgggtggaaca | 125 |
| Reverse primer | ccgacggtagacgcacat | |
| Probe | tgtattgtgccaaggaccagccg | |
| Rpl19 | ||
| Forward primer | ggaagcctgtgactgtccat | 101 |
| Reverse primer | ggcagtacccttcctcttcc | |
| Probe | aagggcaggcatatgggcat |