Table 1.
IgM hybridoma molecular characterization. IGHV, IGHD and IGHJ gene and allele use, % IGHV gene homology to germline V gene (closest allele), CDR3-IMGT length and numbers of N addition are shown.
| Subject | mAb | Vh gene | % gene | D gene | Jh gene | CDR3 length | N addition |
|---|---|---|---|---|---|---|---|
| A | AbA6 | 3–53 | 100 | 6–19 | 6b | 19 | 15 |
| B | DM17 | 3–21 | 100 | 5–5 | 6b | 18 | 4 |
| Bcl | 3–21 | 100 | 3–09 | 6c | 27 | 31 | |
| C* | CbH4 | 3–64 | 100 | 6–13 | 6b | 25 | 24 |
| E* | 6b1A7 | 4–39 | 100 | 3–22 | 4b | 21 | 40 |
| 6b5D7 | 1–69 | 100 | 6–6 | 6b | 14 | 2 | |
| 6b5B12 | 1–02 | 100 | 1–7 | 6b | 19 | 8 |
*
Partial description previously [17]. mAb: monoclonal antibodies.