Fig. 2.

Tissues from mice fed analogue or control diets for 31–33 weeks were fixed in Telly's fixative, paraffin-embedded and sectioned at 5 μm. Sections were stained with haematoxylin and eosin (H&E). (a–c) Femur diaphysis × 100. Bone cortex was thickened by irregular deposition of woven bone. Compared to control-fed animals, the cortex was approximately double and triple the thickness for 1,25D3- and 1alphaD3-fed mice, respectively. (d–f) Tracheal ring cartilage × 200. Expansion of the cartilage ring by irregular nodules of well-differentiated osteoid. Compared to control-fed animals, cartilage rings were mildly and severely thickened and deformed by osteoid deposition in 1,25D3- and 1alphaD3-fed mice, respectively. (g–i) Renal tubules × 600. Mineral deposition along the basement membranes of distal tubules. Occasional mineral deposits were observed along the basement membranes of distal tubules in 1alphaD3-fed mice but rarely found in 1,25D3-fed mice.