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Cytokine secretion by separated ex vivo dendritic cells (DC) either unstimulated or stimulated via CD40 or Toll-like receptor ligation. Separated myeloid DC (mDC) and plasmacytoid DC (pDC) from eight patients and controls each were stimulated in vitro with the indicated stimuli; after 48 h the indicated cytokines were determined in culture supernatants by enzyme-linked immunosorbent assay (interferon-α) or cytokine bead array.
