Fig. 2.

Mechanism of regulation of HLA-DR and CD86 expression in long-term, interleukin (IL)-10-treated monocytes. Negatively separated monocytes were cultured for 7 days with or without (control) IL-10. (a) The mRNA expression of HLA-DR and CD86 was analysed by quantitative polymerase chain reaction. Data from six donors are presented as the fold expression of the house-keeping gene hypoxanthine phosphoribosyl-transferase 1 (HPRT). (b) Seven-day cultured monocytes were analysed flow cytometrically with respect to surface CD86 expression paralleled by intracellular measurement of CD86 protein expression. Individual data from three donors are shown (*P < 0·05 by Wilcoxon matched-pairs signed-ranks test).