Table 2.
CD3+ conditioned media (CM) stimulate fibroblast-mediated collagen gel contraction.
| Exp. | Fibroblasts | Fibroblasts + CD3+ CM* |
|---|---|---|
| 1 | 36·3 ± 0·9 | 4·5 ± 0·1 |
| 2 | 35·1 ± 1·3 | 5·7 + 1·1 |
| 3 | 30·5 ± 0·7 | 9·4 ± 0·5 |
| 4 | 40·8 ± 0·7 | 30·9 ± 1·3 |
| 5 | 40·8 ± 0·7 | 1·5 ± 0·04 |
| 6 | 45·5 ± 0·2 | 19·7 ± 4·9 |
| 7 | 51·3 ± 0·1 | 2·0 ± 0·3 |
| 8 | 40·0 ± 2·7 | 1·4 ± 0·2 |
| 9 | 24·9 ± 0·2 | 1·0 ± 0·1 |
| 10 | 33·2 ± 0·4 | 0·6 ± 0·1 |
| Median | 38·2 | 3·3*** |
| (p25–p75) | (31·9–43·2) | (1·2–14·6) |
Values are percentage of initial gel area as mean ± standard error of the mean of triplicate gels from 10 individual experiments at day 4. Median (p25–p75) of all experiments is presented.
*
CD3+ CM was diluted to 1/5 in serum free Dulbecco's modified essential medium.
***
P < 0·0001 versus fibroblasts alone (Mann–Whitney).