Fig. 1.

Aspergillus (Asp) f16-specific cytotoxic lymphocyte (CTL) activity in lines primed with complete peptide pool pulsed pentadecapeptides-dendritic cells (PPC-DC) compared to lines primed with PPC-DC followed by PPC-B lymphoblastoid cell lines (BLCL). Non-adherent peripheral blood mononuclear cells from donor RD0601 or RD0604 were primed with Asp PPC-DC for five rounds weekly, then split into two parts and primed subsequently with either PPC-DC (denoted as DC-primed) or with PPC-BLCL (denoted as DC/BLCL primed) weekly. CTL activity to PPC-pulsed and non-pulsed BLCL targets was tested 1 week following each round of stimulation. Shown are the mean and standard deviation of the percentage of specific lysis by triplicate wells of effector cells from the two donors against PPC pulsed autologous BLCL (effector : target ratio = 50 : 1). Lysis of non-pulsed targets was consistently below 10% (not shown).