Fig. 3.

Identification of the T cell subset producing interferon (IFN)-γ and mediating cytotoxic lymphocyte (CTL) activity in response to Aspergillus f16 complete peptide pool pulsed pentadecapeptides (PPC). IFN-γ-producing cells stimulated by Aspergillus complete peptide pool were detected by intracellular staining after gating on CD4⁺ and CD8⁺ T cells from dendritic cells (DC)-primed lines and DC/B lymphoblastoid cell lines (DC/BLCL)-primed lines from donors RD0601 and RD0604. Shown are the dot plots of CD69 (y axis) versus IFN-γ (x axis) from the activated CD4⁺ or CD8⁺ T cells. Little or no IFN-γ was detected in non-stimulated lines (not shown) (a). CTL assays were performed using unseparated (solid bars), CD4⁺-enriched (open bars) and CD8⁺-enriched (hatched bars) cells from the DC-primed lines and DC/BLCL-primed lines from donors RD0601 and RD0604. CD4⁺ and CD8⁺ T cells were enriched by negative selection. Shown are the percentage of specific lysis of non-pulsed (NP) BLCL and Aspergillus complete peptide pool pulsed (PPC) BLCL targets by the separated CD4⁺ and CD8⁺ T cells (effector : target ratio = 50 : 1) (b).