Fig. 5.

Surface antigen expression on Aspergillus (Asp) f16 complete peptide pool pulsed pentadecapeptides-pulsed mature dendritic cells (PPC-DC) and PPC-B lymphoblastoid cell lines (BLCL) and cytokine secretions of the pulsed DC and BLCL. Mature DC and Epstein–Barr virus (EBV)-transformed BLCL from donor RD0601 and RD0604 were pulsed with Aspergillus fumigatus complete peptide pool for 2 h, and then incubated at 37°C overnight. The next day, cells were collected by centrifugation, stained with conjugated antibodies and analysed by flow cytometry. Supernatants were saved for cytokine detection later by Bio-Plex cytokine assay (Bio-Rad). Mature DC (open bars) or BLCL (solid bars) were pulsed with Asp f16 A. fumigatus complete peptide pool overnight then stained for cell surface antigens. The results are shown as the percentage of cells expressing the indicated antigen. Data represents mean ± standard deviation (s.d.) of two experiments (a). Supernatants from the PPC-pulsed DC (open bars) or BLCL (solid bars) were tested for cytokine production by Bio-Plex cytokine assay. Cytokine concentrations (pg/ml) are mean ± s.d. of three independent tests (each performes in duplicate) (b).