Fig. 6.

Analysis of the effects of interleukin (IL)-10 and tumour necrosis factor (TNF)-α on the induction of cytotoxic lymphocyte (CTL) responses. T cells from donor RD0601 after fifth-round priming were restimulated with Aspergillus f16 complete peptide pool pulsed pentadecapeptides-dendritic cells (PPC-DC), PPC-B lymphoblastoid cell lines (BLCL), PPC-BLCL in the presence of TNF-α neutralizing antibodies and PPC-BLCL in the presence of IL-10 neutralizing antibodies separately. After 1 week of incubation, the cytolytic activity of all the T cell lines was assessed by chromium release assay. NP-BLCL: non-pulsed BLCL, PPC-BLCL: Asp f16 peptide complete pool pulsed BLCL. Shown are the percentages of specific lysis of BLCL by the lines (effector : target ratio = 50 : 1).