Table 1.
The percentage (%) of CD3+, CD4+ and CD8+ T cells in lines from donors RD0601 and RD0604.
| RD0601 | RD0604 | ||||||
|---|---|---|---|---|---|---|---|
| Priming | Cell lines | CD3+ | CD4+ | CD8+ | CD3+ | CD4+ | CD8+ |
| Fifth | DC primed | 88·9 | 98·5 | 14·0 | 97·6 | 34·5 | 60·0 |
| Sixth | DC primed | 99·0 | 94·3 | 10·9 | 97·7 | 75·0 | 29·1 |
| DC/BLCL primed | 99·2 | 94·7 | 12·3 | 96·7 | 67·1 | 35·0 | |
| Seventh | DC primed | 95·2 | 87·2 | 13·8 | 99·7 | 93·5 | 11·0 |
| DC/BLCL primed | 98·8 | 85·4 | 20·9 | 99·9 | 82·5 | 19·1 | |
| Eighth | DC primed | 98·0 | 90·6 | 13·3 | 99·9 | 95·2 | 6·5 |
| DC/BLCL primed | 90·0 | 76·0 | 21·7 | 96·2 | 98·8 | 5·0 | |
Immunophenotype was performed by flow cytometry following the indicated number of primings using dendritic cells (DC) alone or DC followed by B lymphoblastoid cell lines (BLCL) as antigen-presenting cells (APC) (DC/BLCL). The results are the percentage of antigen-positive cells in the cultures. Fifth, sixth, seventh and eighth denote weekly stimulation with Asp f16.