Fig. 2.

Enhanced cytokine/CD40L production by CD2-activated lamina propria T lymphocytes (LPT). Isolated peripheral blood T lymphocytes (PBT) and LPT were activated by CD2 monoclonal antibody (mAb) M1 (1 μg/ml), M2 (1 μg/ml) and 3PT (0·33 μg/ml). (a) After 4 h, interleukin (IL)-2 mRNA expression was determined by quantitative reverse transcribed polymerase chain reaction. Culture supernatant was harvested after 24 h, and the IL-2 content was determined by enzyme-linked immunosorbent assay (detection limit 16 pg/ml). Results are representative of at least three independent experiments. (b) Ratio cytokine (and CD40L) mRNA expression of CD4⁺ LPT/CD4⁺ PBT after CD2 stimulation for 4 h (box plot diagrams showing median, 25th and 75th percentile, minimum and maximum; n = 5). (c) Time–course of CD2-induced IL-2 and CD40L mRNA expression in PBT and LPT. Results are representative of three independent experiments.