Table 3.
Dilution linearity of samples with high vascular endothelial growth factor (VEGF) levels.*
| Sample 1 | Sample 2 | Sample 3 | ||||
|---|---|---|---|---|---|---|
| Dilution factor | Expected | Observed | Expected | Observed | Expected | Observed |
| None | n.a. | 25·66 | n.a. | 240·45 | n.a. | 677·30 |
| ×2 | 12·83 | 13·01 | 120·23 | 121·62 | 338·65 | 330·60 |
| ×4 | 6·42 | 6·41 | 60·11 | 60·45 | 169·33 | 165·34 |
| ×8 | 3·21 | 2·31 | 30·06 | 32·81 | 84·66 | 86·09 |
| ×16 | 1·60 | 1·80 | 15·03 | 14·11 | 42·33 | 36·04 |
| ×32 | 0·80 | 1·77 | 7·51 | 6·89 | 21·17 | 22·34 |
*
We conducted a regression analysis of expected (x) versus measured (y) values (in units per millilitre). Respective slopes, intercepts and r-values were 0·98187, 0·17814 and 0·99063 for sample 1; 1·01366, −0·04837 and 0·99956 for sample 2; and 0·97813, −0·27582 and 0·99967 for sample 3; n.a.: not applicable.