Fig. 3.

Influence of imatinib (IM) on CDK mRNA expression. Purified CD4⁺ T cells were stimulated for 3 days with phorbol myristate acetate/ionomycin in the presence or absence of 10 μM IM. Total RNA was isolated and subjected to RNAse protection assay as described in Materials and methods. Total RNA, isolated from unstimulated CD4⁺ T cells, was included in each experiment. The results of a representative experiment (n = 3) is depicted.