Fig. 4.

Influence of imatinib (IM) on T cell activation markers. Peripheral blood mononuclear cells were stimulated for 3 days with plate-bound anti-CD3/anti-CD28. The cells were cultured in the presence or absence of 10 μM IM. On day 3, the cells were stained for CD4, CD45RO and CD25 and analysed by fluorescence activated cell sorter analysis. CD4⁺ T cells were gated and displayed as dot-blots for the expression of CD25 and CD45RO. Note that in the presence of IM conversion from CD45RO^– cells to CD45RO⁺ cells is inhibited and that the expression of CD25 in both subsets is decreased. The results of a representative experiment (n = 3) are depicted.