Fig. 5.

Influence of imatinib (IM) on cytokine production. (a) Peripheral blood mononuclear cells were stimulated for 4 h with phorbol myristate acetate (PMA)/ionomycin and stained for interleukin-2 in CD4⁺CD45RO^– and CD4⁺CD45RO⁺ cells. (b) Purified CD4⁺ T cells were stimulated for 7 days with either anti-CD3/anti-CD28 or PMA/ionomycin. The cells were cultured in the presence or absence of 10 μM IM. On day 7, supernatants were harvested and interferon (IFN)-γ production was assessed by enzyme-linked immunosorbent assay. In (b) a total of four different experiments were performed. The results are expressed as mean IFN-γ production (pg/ml/10⁶ cells) ± standard deviation.