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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Dec 15;1777(2):227–237. doi: 10.1016/j.bbabio.2007.11.011

Fig. 3.

Fig. 3

Line broadening of Trp ε-imino indole signals by PROXYL spin label attached at Cys223 of the subunit a. (A) Control spectrum of wild type protein recorded after reducing the PROXYL spin label with phenylhydrazine. Resonance assignments are indicated by the arrows. (B) PROXYL labeled wild type sample showing loss of W231, W232, W235 and W241 resonances. (C) Overlay of the spectra of the spin-labeled W186F variant of subunit a (black) and of the phenylhydrazine reduced sample [red].