Figure 3. Effect of secretagogues on jejunum epithelia from WT and Kcnn4 null mice.

All details for the addition of agonists and blockers are as in Fig. 1. When jejunum of WT mice (A) was challenged with 100 μm serosal carbachol, there were negative changes in V_te that were partially blocked by 3 μm clotrimazole and fully blocked by additional 5 mm Ba²⁺. B shows results for a jejunum from Kcnn4 null mouse, where carbachol addition did not produce the negative changes related with anionic secretion. C, summary of ΔI_sc values for a series of experiments performed on jejunum epithelium from wild-type and Kcnn4 null mice. Data are means ±s.e.m. of 5 individual experiments for each animal. A P < 0.0005 level of significance for the difference with the effect of carbachol on WT tissue is indicated by *. None of the effects of carbachol on Kcnn4 KO tissue was significantly different from zero.