Table 3.
Effects of i.c.v Ucn 2 on 7 h prandial intake in genetically selected DIO and DR rats
| Parameter | 0–2 h | 2–7 h | 0–7 h | |||
|---|---|---|---|---|---|---|
| DR | DIO | DR | DIO | DR | DIO | |
| Feeding | ||||||
| Intake (g) | ||||||
| ANOVA | — | — | ## | # | # | ## |
| Linear contrast | — | † | †† | †† | †† | — |
| 0 μg | 4.6 ± 0.6 | 6.4 ± 0.9 | 6.8 ± 0.5 | 5.0 ± 0.5 | 11.4 ± 0.9 | 11.4 ± 0.6 |
| 0.3 μg | 5.1 ± 0.7 | 3.7 ± 0.7 | 3.7 ± 0.7** | 4.2 ± 0.6 | 8.8 ± 0.8 | 10.3 ± 1.3 |
| 1 μg | 3.8 ± 0.6 | 3.7 ± 0.8 | 3.7 ± 0.8** | 3.8 ± 0.6 | 7.5 ± 0.8* | 7.9 ± 1.2* |
| 3 μg | 3.6 ± 0.3 | 3.6 ± 0.9 | 3.6 ± 0.9* | 2.7 ± 0.5* | 7.2 ± 1.1* | 7.1 ± 1.1* |
| Duration (min) | ||||||
| ANOVA | — | — | ## | — | # | # |
| Linear contrast | — | † | †† | — | †† | — |
| 0 μg | 20.0 ± 2.6 | 25.0 ± 3.1 | 30.7 ± 2.4 | 20.2 ± 3.5 | 50.7 ± 3.9 | 45.1 ± 3.7 |
| 0.3 μg | 23.0 ± 2.5 | 23.6 ± 2.6 | 19.1 ± 4.4** | 15.6 ± 2.7 | 42.1 ± 4.7 | 39.2 ± 3.8 |
| 1 μg | 18.0 ± 3.5 | 17.1 ± 3.8 | 18.4 ± 4.0* | 16.9 ± 2.5 | 36.4 ± 5.4* | 34.0 ± 3.5 |
| 3 μg | 17.3 ± 1.4 | 17.4 ± 4.5 | 16.7 ± 3.7** | 14.0 ± 1.9 | 34.1 ± 4.1* | 31.4 ± 3.8* |
| Drinking | ||||||
| Intake (ml) | ||||||
| ANOVA | # | — | ## | # | ## | # |
| Linear contrast | †† | — | ††† | †† | ††† | †† |
| 0 μg | 3.9 ± 1.2 | 4.8 ± 0.6 | 11.6 ± 2.2 | 10.1 ± 1.0 | 7.7 ± 1.3 | 5.3 ± 0.7 |
| 0.3 μg | 3.4 ± 1.0 | 4.8 ± 0.9 | 8.1 ± 1.8* | 9.7 ± 1.3 | 4.7 ± 1.2 | 4.9 ± 0.9 |
| 1 μg | 2.2 ± 0.7 | 3.3 ± 1.0 | 5.5 ± 1.4** | 6.5 ± 1.7 | 3.3 ± 1.0** | 3.2 ± 1.1 |
| 3 μg | 1.0 ± 0.2* | 3.6 ± 1.4 | 3.9 ± 1.0** | 5.6 ± 1.5 | 2.9 ± 0.8** | 2.0 ± 0.7 |
| Duration (min) | ||||||
| ANOVA | — | # | # | — | ## | ## |
| Linear contrast | † | † | †† | † | †† | †† |
| 0 μg | 7.8 ± 2.0 | 11.4 ± 2.3 | 11.8 ± 2.0 | 15.3 ± 2.6 | 19.6 ± 3.5 | 26.7 ± 3.7 |
| 0.3 μg | 5.9 ± 1.6 | 15.7 ± 2.6 | 6.8 ± 1.7* | 10.5 ± 1.9 | 12.7 ± 2.6 | 26.2 ± 3.1 |
| 1 μg | 5.9 ± 1.4 | 8.0 ± 1.8 | 5.6 ± 1.8* | 8.1 ± 3.0 | 11.5 ± 1.8 | 16.1 ± 2.7 |
| 3 μg | 2.7 ± 1.0 | 6.7 ± 3.2 | 4.7 ± 1.2* | 5.1 ± 2.2 | 7.4 ± 1.8** | 11.8 ± 3.1* |
Effect of third ventricle Ucn 2 treatment on nocturnal prandial ingestion of genetically selected diet-induced obesity-resistant (DR) and susceptible (DIO) rats. Data express the mean ± s.e.m. quantity or duration of food and water intake within meals of adult male DR (n = 9) and DIO (n = 8) rats during the first 7 h of the dark cycle following Ucn 2 pretreatment. Subjects were pretreated (−10 min) with Ucn 2 in a balanced Latin square design with test sessions beginning at the onset of the dark cycle. Symbols denote significant differences:
P < 0.05
P < 0.01 from the respective vehicle condition (within-subject Newman–Keuls test)
P < 0.05
P < 0.01 denote a significant main effect of Ucn 2 (ANOVA)
P < 0.05
P < 0.01
P < 0.001 denote a significant log-linear, dose-dependent effect of Ucn 2 (Linear contrast ANOVA).