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. 2007 Jul 12;583(Pt 2):767–784. doi: 10.1113/jphysiol.2007.138024

Figure 1. CS1-null mutation is not lethal and no compensatory increase of CS2 is detectable.

Figure 1

A, the SMCALSE1 gene trap allele was generated, using random insertional mutagenesis with retroviral vector VICTR24. The precise genomic insertion site of the gene-trap vector (asterisk) was determined by inverse genomic PCR. B, the CS1-null mice do not shown any significant behavioural alteration under standard housing conditions. C, Western blot analysis of total homogenates prepared from limb muscles shows that CS1 is missing in CS1-null muscle (right lane). D, representative Western blots of EDL and soleus homogenates from WT and CS1-null mice: lack of CS1 is confirmed and no sign of compensatory increase of CS2 expression is detectable.