Figure 7. RyR expression, but not CS2, SERCA1, and triadin, is upregulated in EDL muscle.

A, binding of ³H-ryanodine shows that the B_max is approximately doubled in CS1-null mice samples as compared to WT samples, whereas the K_d is virtually unchanged. Each data point represents the average of duplicate determinations. B_max and K_d values are calculated by Scatchard plot analysis with Enzifitter version 1.03 Elsevier Biosoft program. B–D) quantitative analysis of total homogenates from WT and CS1-null EDL muscles (left panels) and graphical representation of the mean values of densitometric signals (right panels, arbitrary units). Data plotted in the bar graph are expressed as means ± s.d. for n = 4 WT EDL and n = 4 CS1-null EDL. Densitometric analysis shows a decrease of expression of CS2 in CS1-null EDLs, whereas SERCA1 and 95 kDa triadin contents are not significantly changed.