Figure 4. Coronal sections illustrating different expression of the Y₁R/LacZ transgene in the rostral level of the dorsomedial (DMH) nucleus of male mice from different experimental groups.

A, standard chow-fed male mouse; B, standard chow-fed male mouse treated for 3 days with 1 μg g⁻¹ of murine recombinant leptin; C, MHF diet-fed male mouse; D, MHF diet-fed male mouse treated for 3 days with 1 μg g⁻¹ of murine recombinant leptin. Pictures were digitized as described in Fig. 3. The AOI was drawn following the boundaries of the dorsomedial nucleus (DMH) on the green filter-digitized image. VMHdm, ventromedial hypothalamic nucleus, pars dorsomedialis. * indicates third ventricle; scale bar, 400 μm.