Figure 8. Effects of repeated application of adrenaline on facial, C4 and L1 activities in Atp1a2^−/− mice.

A, the initial application of 0.5 μm adrenaline after starting superfusion induced a high-frequency rhythmic burst in the facial nerve but not in C4. B, high-frequency bursts in both nerves are induced during the fifth 10 min application of adrenaline with 10 min intervals between applications. Ca, faster sweep representation of facial and C4 nerve activities at the black bar in B. Cb, averaged facial nerve activity triggered by C4 (10 cycles). Note that the facial nerve burst precedes the C4 inspiratory burst. D, facial, C4 and L1 nerve activities during the third application of 0.5 μm adrenaline. Note that L1 activity appeared during the preinspiratory phase. VII, facial nerve activity; C4, C4 inspiratory activity; L1, first lumbar ventral root activity.