Table 1.

Ionic composition of the solutions

Solution	MgCl2	Na2ATP	EGTA	HDTA	CaEGTA	KProp
Relaxing	8.37	5.80	20	0	0	42.5
Pre-activating	7.78	5.80	0.5	19.5	0	43.6
Activating	7.63	5.87	0	0	20	43.6

Concentrations are shown in mm. Potassium propionate (KProp) was prepared by dissolving equimolar amounts of propionic acid with KOH. CaEGTA was made by dissolving equimolar amounts of CaCO₃ and EGTA followed by boiling of the solution to remove CO₂. At each temperature the pH of the solutions was adjusted to 7.1 with KOH.