Skip to main content
. 2007 Aug 30;584(Pt 2):637–650. doi: 10.1113/jphysiol.2007.141457

Figure 1.

Figure 1

Anti-pMyoDT115 antibody is specific for MyoD phosphorylated at T115 A, spots of phosphopeptide, but not dephosphopeptide, were clearly detected down to < 10 ng. B, Western blot of nuclear extracts from Hek-293 cells after transfection with pCMS-EGFP expressing EGFP only (sham), pCMS-EGFP-MyoDwt expressing wild type MyoD, pSP3-MyoD expressing MyoD with a S200A mutation, or pCMS-EGFP-MyoDA115 expressing MyoD with a T115A mutation. The blots were stained with anti-MyoD or anti-pMyoDT115. Loading was adjusted for differences in transfection efficiency by adjusting the load to yield equal signals with anti-MyoD.