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. 2007 Aug 30;584(Pt 2):637–650. doi: 10.1113/jphysiol.2007.141457

Figure 4.

Figure 4

MyoD and myogenin mRNA and protein levels change in response to electrical activity A, the level of mRNA for MyoD and myogenin quantified by RT-PCR relative to expression of the housekeeping gene β2-microglobulin (Myog). Some of the RT-PCR data were confirmed on Northern blots probed against MyoD and myogenin (upper panel). B and C, the level of MyoD and myogenin proteins in nuclear extracts quantified on Western blots stained with anti-MyoD or anti-myogenin antibodies. Examples of representative Western blots are shown above graphs. Data are given as the ratio between the levels in EDL muscles stimulated with a slow (S-stim) pattern, and contralateral normal EDLs (A and B), or as the ratio between fast-stimulated (F-stim) soleus muscles and contralateral normal solei (C). Data are given as mean ±s.e.m. of the stimulated versus normal muscles on a logarithmic scale, n= 6–10. *P≤ 0.05; **P≤ 0.01. Numerical values are given in Table S1.