Table 2.
Relative expression of RAS component mRNAs in enterocytes isolated from rat jejunum and ileum as determined by real-time RT-PCR
| Gene | CTa | ΔCTb | ΔΔCTc | Expression relative to jejunum (fold change)d |
|---|---|---|---|---|
| Jejunum | ||||
| β-actin | 16.13 ± 0.03 | — | — | — |
| AT1 | 25.31 ± 0.02 | 9.18 ± 0.05 | — | — |
| AT2 | 26.37 ± 0.01 | 10.24 ± 0.04 | — | — |
| AO | 26.99 ± 0.02 | 10.86 ± 0.05 | — | — |
| ACE | 18.58 ± 0.02 | 2.45 ± 0.05 | — | — |
| Distal Ileum | ||||
| β-actin | 15.60 ± 0.04 | — | — | — |
| AT1 | 22.93 ± 0.02 | 7.33 ± 0.06 | −1.85 ± 0.11 | 3.61 |
| AT2 | 24.14 ± 0.01 | 8.54 ± 0.05 | −1.7 ± 0.09 | 3.25 |
| AO | 24.73 ± 0.02 | 9.13 ± 0.03 | −1.73 ± 0.08 | 3.31 |
| ACE | 20.68 ± 0.01 | 5.08 ± 0.05 | 2.68 ± 0.10 | 0.16 |
a
The average CT data for each sample
b
The ΔCT value is calculated by subtraction of the β-actin CT from each sample CT
c
The ΔΔCT value is calculated by subtraction of the control ΔCT from each sample ΔCT
d
The expression relative to control is calculated using the expression 
.