Figure 8.

CaBP antibody labelling in chicken basilar papilla Antibodies available for CaBP1 and CaBP4 were used to label cryosections (across the long axis) of the chicken basilar papilla. Tegmentum vasculosum (collapsed onto basilar papilla) is labelled ‘TV’. Tectorial membrane (visible only in A) is labelled ‘TM’. A single hair cell is shaded white in each image for reference. A, antiserum to CaBP1 (green) bound to hair cells and supporting cells in the basilar papilla, to the tectorial membrane, and to cells in the tegmentum vasculosum. Immunolabelling of hair cells was concentrated in the cuticular plate and hair bundle. Antibody to choline acetyl-transferase (ChAT, red) indicates the position of efferent cholinergic endings located on the synaptic pole of hair cells. B, antiserum to CaBP4 (green) strongly labelled hair cells in the basilar papilla, and TV very faintly. Hair cell cytoplasm labelled as strongly as cuticular plate (hair bundles did not label). Each image has been optimized for brightness and contrast, so relative intensities cannot be compared between panels. ChAT antibody label (in red) indicates efferent boutons at synaptic pole of hair cells.