Figure 10.

Lung epithelial cells are reservoirs for murine gammaherpesvirus-68 (γHV68) replication and CCL2 production. Epithelial cells were purified from mice on Day 21 post–FITC + γHV68 treatment. Isolated cells were plated on fibronectin-coated eight-chambered glass slides and fixed, and immunofluorescent staining was performed. (A) Staining with anti-γHV68 polyclonal antisera detected with a phycoerythrin (PE)-conjugated secondary antibody. (B) Colocalization of γHV68 staining (red, PE) with anti-CCL2 (Cy5, blue) at ×200 original magnification and (C) a different section of this slide at ×400 original magnification.