Figure 4.

SOCE during Ca²⁺ release and removal A, selected images of fluorescence F₃ of cytosolic rhod-2 and B, ratio R of fluorescence images F₁ and F₂ of mag-indo-1 in the t system, simultaneously acquired while applying the release-inducing low Mg²⁺ solution. C, spatially averaged values of F₃ (normalized by resting value F_3,0, red) and R (blue) versus elapsed time (which maps to the abscissa of x–y scans as described in Methods). The interval of solution change is indicated by the black bracket in image B at 3 s, and by the light blue bar in C, where fluorescence and ratio plots were omitted during solution change. (ID: 072005b_s013.)