SOCE with suppressed change in [Ca2+]c A, F3 and B, R in t system, acquired while changing from a solution with 5 mm BAPTA, 0 Ca and 0.65 mm Mg2+ to release-inducing low Mg2+–BAPTA. C, spatially averaged F3/F3,0(red) and R (blue) versus elapsed time, which directly correspond to time and y axis in A and B. Inset, average R in central 40 μm (‘middle') and 30 μm slice starting at the lower edge (‘edge') of the fibre image. Note the periodic pattern of [Ca2+]t-sys in C, which results from the regular placement of t-tubules along the fibre. Other details as in Fig. 4. (ID: 072805f_s012.)