Figure 2.

PDK2 and 4 mRNA and protein expression in skelatal muscle A, the relative expression of pyruvate dehydrogenase kinase (PDK) 2 and 4 mRNAs measured in soleus muscle of rats fed with either vehicle (hydroxypropylmethylcellulose) or GW610742 at 5 mg (kg bm)⁻¹ (cross-hatched columns) and 100 mg (kg bm)⁻¹ (open columns). The values are expressed as 2^−ΔΔC_t normalized to endogenous cyclophilin. The vehicle group was used as calibrator with a value of 1. *Significantly different from the corresponding control (vehicle) group (P < 0.05). B, the average density of pyruvate dehydrogenase kinase PDK4 protein bands, measured by Western blot in soleus muscle of rats fed with either vehicle (hydroxypropylmethylcellulose) (filled columns) or GW610742 at 5 mg (kg bm)⁻¹ (cross-hatched columns) and 100 mg (kg bm)⁻¹ (open columns). *Significantly different from the corresponding control (vehicle) group (P < 0.05). C, a representative Western blot of PDK4 protein detected at 46 KDa.