Figure 6.

Hypothalamic IL-1β mRNA induction after TURP is attenuated GD 18-females A, similar to other central markers of inflammation, IL-1β mRNA expression increased in the hypothalamus of NP females, 11 h after TURP injection (100 μl; i.m.). In TURP-injected GD 18 dams the levels of this mRNA were lower, but still significantly induced. TURP-injected NP females versus SAL-injected NP females: **P < 0.01; TURP-injected GD 18 females versus SAL-injected GD 18 females and versus TURP-injected NP females: *P < 0.05. Each band in the gel represents one animal; two animals per group are being shown. B, top panel, hypothalamic IL-1β mRNA levels (x axis) correlated significantly with temperature 11 h after TURP injection (y axis) in NP females (n = 12 including SAL- and TURP-injected animals; r²= 0.77, P = 0.0002). Bottom panel, the same analysis showed that no such correlation existed in GD 18 dams (n = 11; r²= 0.16, P = 0.22). C, top panel, as in B, in NP females hypothalamic IL-1β mRNA levels (y axis) showed a low, but significant correlation with plasma IL-6 levels (x axis) (n = 12 including SAL- and TURP-injected animals; r²= 0.45, P = 0.017). Bottom panel, in GD 18 dams this correlation was still present (n = 11; r²= 0.46, P = 0.046). The expression of microsomal PGE synthase-1 is attenuated in GD 18 dams after TURP injection.