CHEF separation of chromosomes from C. albicans strain SC5314 and from C. dubliniensis strain CD36. Left: optimal separation conditions for the smaller chromosomes. Center: CHEF separation of the same strains under conditions optimal for the large chromosomes. Because the position of chromosome R on a pulse-field gel is variable, band XI (one homologue of chromosome R) sometimes runs ahead of band X (chromosome 2, as it does in this gel. The numbers and letter on the left identify the chromosomes of SC5314; the Roman numeral labels on the figure refer to the bands in the CD36 lane. Right: Location of several probes on the SC5314 and CD36 karyotypes. Probes: A, rDNA (SfiI fragments RS/RU); B, HCS1 (RB); C, p2A (2A); D, CPY52 (4F); E, MTLα (5I); F, MTLa (5I); G,HEX1 (5I); H, HIS1 (5I); I, p282 (7A).