Skip to main content
. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 28;368(2):285–291. doi: 10.1016/j.bbrc.2008.01.088

Figure 1. Characterization of the Wnt7a-Fzd5 Interaction Using Purified Protein.

Figure 1

(A) Wnt7a directly interacts with the Fzd5 CRD. Ishikawa cell extracts from and extracts from cells stably overexpressing Wnt7a V5/His were immunoprecipitated with Fzd5CRD-Fc or Fc. (B) Negative controls for ELISA-based protein-protein binding assay indicating a minimal amount of background signal compared to maximum signal detected for Wnt7a and Fzd5CRD-Fc binding. ***p≤0.001 compared to all controls using ANOVA and Bonferroni post-hoc analysis (C) Wnt7a-Fzd5CRD-Fc binding curve and (D) scatchard plot obtained using non-linear regression analysis in GraphPad Prism. The calculated Bmax and Kd were 5.3 nM and 75.4 nM, respectively.