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. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 28;368(2):285–291. doi: 10.1016/j.bbrc.2008.01.088

Figure 4. Purified Wnt7a Induces Reconstituion of split-eGFP and Activates βcatenin/Canonical Wnt Signaling.

Figure 4

(A) Stable xDshN/Fzd5C cells and null cells were seeded on coverslips and treated with purified Wnt7a (300 ng/ml). Cells were fixed 24 hours post-treatment and images were collected via fluorescence microscopy at 400 x magnification. (B) Purified Wnt7a activates TopFlash in xDshN/Fzd5C cells. Luciferase activity was measured 24-h post-transfection. Results are representative of at least three separate experiments performed in duplicate ± S.E. * and **, indicate (p≤0.05) and (p≤0.01) based on ANOVA and Bonferroni post-hoc for multiple comparisons.