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| Experimental Method | Kam (pKam) | Ka2m pKa2m) |
|---|---|---|
| Membrane Permeability Studies | 1.08 ± 0.17 × 10−8 (7.97 ± 0.16) | 1.53 ± 0.64 × 10−9 (8.81 ± 0.42) |
| Membrane Partitioning Studies | 1.95 ± 0.32 × 10−8 (7.71 ± 0.16) | 1.1 ± 0.6 × 10−9 (8.95 ± 0.54) |
| Fluorescence Spectroscopic Studies | N/A | 5.8 ± 1.1 × 10−10 (9.23 ± 0.19) |
Ionization constants (mean ± std) of DB-67 when bound to the gel phase (DSPC) bilayer, measured using various methods
