Figure 4.

Quantification of the percentage of Pkd1^del2-11 allele by eMLPA. To validate the eMLPA strategy, the percentage of Pkd1^del2-11 allele is shown for eight Pkd1^{del2-11, lox} controls with an expected value of 50%, and for a dilution series ranging from a hundred to zero percent of Pkd1^del2-11 allele, obtained by diluting Pkd1^{del2-11, del2-11} with Pkd1^{lox, lox} DNA at different ratios. All controls and samples from the dilution series gave the expected results within a five percent interval. The percentage of Pkd1^del2-11 allele is also measured in adult or neonatal tamoxifen treated Cre; Pkd1^lox,+, Cre; Pkd1^{del2-11, lox} and Cre; Pkd1^{lox, lox} mice. The error bars represent the standard error of the mean. Hybridizations were performed three times and PCR's were performed in triplicate on each hybridization.