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. 2008 Mar 19;6:11. doi: 10.1186/1477-7827-6-11

Figure 1.

Figure 1

Effect of glucose treatment on basal and FSH- or IGF-1-stimulated progesterone and oestradiol secretions by rat granulosa cells. Granulosa cells from immature rats were cultured for 48 h in medium with serum and then in serum-free medium in the absence or in the presence of 10 g/l glucose ± 10-8 M FSH or 10-8 M IGF-1 for another 48 h (A and B) as described in Materials and Methods. The culture medium was then collected and assayed for progesterone (A) and oestradiol (B) by RIA. Results are means ± SE for three groups of granulosa cells. Each group of granulosa cells was obtained from about 30 rats. Bars with different letters are significantly different (p < 0.05). The letter "a" indicates values which are not significantly different from control (without FSH or IGF-1 and glucose).