Table 2.
Immunology of single cell suspensions collected from metinel nodes
| Patient | Time in culture (d) | No. of cells at start (millions) | No. of cells at end (millions) | Ratio of cells start/end | Start (% gated), ratio CD4/CD8 and CD19/CD56 | End, ratio CD4/CD8 and CD19/CD56 | Ratio CD4/CD8 start | Ratio CD4/CD8 end |
|---|---|---|---|---|---|---|---|---|
| 1 | 31 | 100 | 80 | .8 | 24/11 and 55/− | 24/16 and −/− | 1.8 | 1.5 |
| 2 | 33 | 42 | 40 | .95 | 51/15 and 13/− | 24 and .12/− | 3.4 | 1.5 |
| 3 | 28 | – | – | – | 8/39 and 1/− | – | .2 | – |
| 4 | 20 | 65.4 | – | – | 37/9 and −/− | – | 4.1 | – |
| 5 | 43 | 130 | – | 1.3 | 47/7.1 and 28/1.3 | 73/22 and .09/.17 | 6.6 | 3.3 |
| 6 | 6 | 5 | – | – | .2/1.6 and .4/.4 | – | .125 | – |
| 7 | 6 | 5 | .7 | .02 | 16.4/6.5 and 24.2/1.6 | – | 2.5 | – |
| 8 | 52 | 42.6 | – | – | 15.9/7.6 and 9/3.4 | – | 2.1 | – |
| 9 | 15 | 12 | – | – | – | – | – | – |
| 10 | 74 | 166.8 | 5 | .03 | 40/7 and .5/.3 | – | 5.7 | – |
| 11 | 35 | 209 | 140 | 9.3 | 12.8/16.3 and 1.9/0 | – | .8 | – |
| 12 | 31 | 285 | 2 | .01 | 16.4/31.8 and .1/0 | – | .5 | – |
| 13 | 24 | 3 | – | – | – | – | – | – |
| 14 | 32 | 30 | 1 | .05 | – | – | – | – |
| 15 | 48 | 36 | – | – | 0/.1 and 0/0 | – | 0 | – |
| 16 | 45 | 20 | – | – | 28.25/6.95 and 1.15/0 | – | 4.1 | – |
| 17 | 20 | 89 | 4 | .04 | 5.8/2.6 and 4.2/.2 | 34.2/30.4 and 7.6/20.1 | 2.2 | 1.1 |
| 18 | 29 | 67 | 4 | .06 | 9.7/3.8 and 1.3/.1 | – | 2.6 | – |
| 19 | 43 | 11 | 15 | 1.36 | 12.7/15.8 and 3/18.9 | 85/3 and .9/8.4 | .8 | 28.3 |