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. 2008 Apr 18;4(4):e1000049. doi: 10.1371/journal.ppat.1000049

Figure 3. Dendritic cells facilitate measles virus infection of T-lymphocytes in clusters that are interconnected.

Figure 3

(A–D) T-lymphocytes (2×105 cells), alone or in the presence of DCs (5×104 cells), were pre-incubated with mannan and infected with MV-IC323-EGFP (TCID50 5×104 unless depicted otherwise) and cultured for 72 hours. (A,B) The infection was analyzed by fluorescence microscopy. The magnification is depicted. (B) DCs or T-lymphocytes were labeled red using the membrane dye PKH26 prior to the experiment and infection by MV-IC323-EGFP is shown by EGFP expression (green). (C,D) The infection of DCs and T-lymphocytes was analyzed by measuring EGFP expression by flow cytometry. Standard deviations represent the standard deviation of triplicates. A representative experiment out of two is shown.