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. Author manuscript; available in PMC: 2009 Mar 10.

Published in final edited form as: Chem Biol Interact. 2007 Nov 19;172(1):27–38. doi: 10.1016/j.cbi.2007.11.003

Fig. 5 — Effect of different ratios of linoleic acid (LA) to α-linoleic acid (ALA) on PCB77-stimulated release of PGE₂ from EC. Cells were exposed to 3.4 μM PCB77 for 6, 18 and 24 h (Fig. 5A). In Fig. 5B, cells were incubated in media supplemented with 20 μM fatty acids, either all LA, all ALA, or different ratios of LA to ALA (R2:1, R1:1, R1:2) for 18 h. Some cultures were first pre-treated with these different ratios of fatty acids for 18 h, followed by co-exposure to 3.4 μM PCB77 for additional 24 h. C = vehicle control. Supernatants of cell cultures were collected and PGE₂ levels were measured by enzyme immunoassay. Bars represent means ± SEM from three independent experiments. Statistical comparisons of the two experimental settings (without or with added PCB77) are indicated by small or capital letters.

Fig. 5 — Effect of different ratios of linoleic acid (LA) to α-linoleic acid (ALA) on PCB77-stimulated release of PGE₂ from EC. Cells were exposed to 3.4 μM PCB77 for 6, 18 and 24 h (Fig. 5A). In Fig. 5B, cells were incubated in media supplemented with 20 μM fatty acids, either all LA, all ALA, or different ratios of LA to ALA (R2:1, R1:1, R1:2) for 18 h. Some cultures were first pre-treated with these different ratios of fatty acids for 18 h, followed by co-exposure to 3.4 μM PCB77 for additional 24 h. C = vehicle control. Supernatants of cell cultures were collected and PGE₂ levels were measured by enzyme immunoassay. Bars represent means ± SEM from three independent experiments. Statistical comparisons of the two experimental settings (without or with added PCB77) are indicated by small or capital letters.