Fig. 6.
Effect of NF-κB inhibitor on PCB77- or linoleic acid (LA)-induced VCAM-1 and COX-2 expression. Cells were exposed to 3.4 μM PCB77 or 20 μM LA alone for 6 h, or first pre-enriched with 50 μM PDTC for 4 h followed by coexposure to PCB77 or LA for additional 6 h. C (PCB77) = vehicle control for PCB77; C (LA) = control for linoleic acid. PCB77- or LA-induced VCAM-1 (Fig. 6A) and COX-2 (Fig. 6B) mRNA levels, as measured by real-time RT-PCR. β-Actin was used as a housekeeping gene. Experiments were repeated three times. Values are means ± SEM. Different letters represent significant difference among treatment groups.