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Effect of individual site mutations on ERα activity. COS-1 cells were co-transfected with ERE-3-TATA-luc, pRL-TK, and the wild-type ERα expression vector (ERα) or versions with alanine or glutamic acid substitutions, as indicated. Cells were treated and luciferase assays carried out as for Fig. 4. A parallel transfection series was assayed for ERα expression by immunoblot (α-ER).
