Skip to main content
. Author manuscript; available in PMC: 2009 Mar 11.
Published in final edited form as: Curr Biol. 2008 Mar 11;18(5):322–330. doi: 10.1016/j.cub.2008.02.005

Figure 2. Cellular Localization of Rga4 Is Regulated by Pom1 Kinase.

Figure 2

(A) Localization of Rga4 during the cell cycle. rga4:GFP cells grown on EMM agarose were observed. Representative projection images are aligned in the order of cell cycle progression. (B) The Δpom1, pom1−2, and Δtea1 mutants show a sock-like localization pattern of Rga4. Rga4GFP was observed in Δpom1, pom1−2, Δtea1, Δbud6, Δtea3, and orb2−34 mutants. Midsection images are shown. Asterisks mark the cell ends from which Rga4GFP was excluded. Arrows indicate dividing cells. (C) Localization of Rga4 is altered by ectopic overexpression of Pom1 kinase. A rga4:RFP CRIB-GFP strain carrying the pREP1-pom1:myc plasmid was grown at 30°C in EMM medium without thiamine to overexpress Pom1 and observed at 14 hr and 16 hr after removal of thiamine. Projection images are shown. CRIB-GFP images of the same fields are presented in Figure S2A. Scale bars represent 5 μm. Images were taken at 0.4 μm steps and deconvolved.